Identification of novel single nucleotide polymorphisms in the DGAT1 gene of buffaloes by PCR-SSCP |
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| Authors: | Ashwin A Raut Anil Kumar Sheo N Kala Vinod Chhokar Neeraj Rana Vikas Beniwal Sundeep Jaglan Sachin K Samuchiwal Jitender K Singh Anamika Mishra |
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| Institution: | 1.High Security Animal Disease Laboratory, Bhopal, Madhya Pradesh, India.;2.Department of Bio & Nano Technology, Guru Jambheshwar University of Science & Technology, Hisar, Haryana, India.;3.Central Institute for Research on Buffalo, Hisar, Haryana, India.;4.Department of Biotechnology, Maharishi Markandeshwar University, Mullana, Ambala, Haryana, India.;5.Indian Institute of Integrative Medicine, Jammu, Jammu & Kashmir, India. |
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| Abstract: | Diacylglycerol O-acyltransferase 1 (DGAT1) is a microsomal enzyme that catalyzes the final step of triglyceride synthesis. The DGAT1 gene is a strong functional candidate for determining milk fat content in cattle. In this work, we used PCR-SSCP (polymerase chain reaction-single-strand conformation polymorphism) and DNA sequencing to examine polymorphism in the region spanning exon 7 to exon 9 of the DGAT1 gene in Murrah and Pandharpuri buffaloes. Three alleles (A, B and C) and four novel single-nucleotide polymorphisms were identified in the buffalo DGAT1 gene. The frequencies of the alleles differed between the two buffalo breeds, with allele C being present in Murrah but not in Pandharpuri buffalo. The allele variation detected in this work may influence DGAT1 expression and function. The results described here could be useful in examining the association between the DGAT1 gene and milk traits in buffalo. |
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| Keywords: | buffalo DGAT1 PCR-SSCP polymorphism SNP |
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