Polypeptide composition of the globin poly(A)-protein complex from rabbit reticulocytes |
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| Authors: | William R Jeffery Cynthia Peters |
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| Institution: | (1) Department of Biophysical Sciences, University of Houston, 77004 Houston, Texas, USA;(2) Present address: Department of Zoology, University of Texas, 78712 Austin, Texas, USA |
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| Abstract: | Polypeptides associated with the rabbit reticulocyte poly(A)-protein complex, isolated by oligo (dT)-cellulose chromatography, were analyzed by SDS-polyacrylamide gel electrophoresis. The complex derived from EDTA released total polysomal mRNP and 20S globin mRNP by RNase treatment contained four polypeptides of molecular weight 58000, 67000, 71000 and 79000. A 79000 molecular weight polypeptide, which was also a major component of the reticulocyte low molecular weight cytoplasmic fraction, was shown to form tenacious associations with poly(A) in vitro. |
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