A serum-free medium for colony growth and hyaluronic acid production by Streptococcus zooepidemicus NJUST01 |
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Authors: | Jianfa Zhang Xia Ding Liuyan Yang Zhiming Kong |
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Affiliation: | (1) State Key Laboratory of Pollution Control and Resource Reuse, Nanjing University, Nanjing, 210093, People’s Republic of China;(2) Department of Biochemical Engineering, Nanjing University of Science & Technology, Nanjing, 210094, People’s Republic of China |
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Abstract: | A hyaluronic acid (HA)-producing strain, Streptococcus zooepidemicus NJUST01, can grow in a serum-free agar medium, with starch as exclusive carbon source, but not glucose, sucrose, dextrine, xylose, or lactose. In this starch medium, the strain NJUST01 reproduced successively at 37°C for 60 generations, with no obvious variation on morphology and physiology, but colonies of the strain after 60th generation could not produce a clear hemolytic zone on sheep blood agar plates. Hyaluronic acid production by the strain NJUST01 was analyzed relative to the starch medium. Employing a multifactor cross experiment, an optimum medium revealed for hyaluronic acid production was composed of 5% starch, 0.3% glucose, 0.5% peptone, 0.15% MgSO4, and 2.0% K2HPO4. The amount of HA 6.7 g/l was obtained in batch fermentation on a rotary shaker at 37°C, 220 rpm for 36 h. |
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