Crystallization of O6-methylguanine-DNA methyltransferase from Escherichia coli |
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Authors: | P C Moody B Demple |
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Affiliation: | Physics Department, Imperial College, London, U.K. |
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Abstract: | The 19,000 Mr C-terminal domain of the Escherichia coli ada gene product that contains O6-methylguanine-DNA methyltransferase DNA repair activity has been crystallized in a low-salt environment. The crystals, which diffract to 2.3 A (1 A = 0.1 nm), are suitable for detailed structural studies. The space group is P21 with unit cell dimensions a = 46.3 A, b = 45.8 A, c = 46.9 A and beta = 113.3 degrees. |
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