Improvement of the ethanol productivity in a high gravity brewing at pilot plant scale |
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Authors: | Dragone Giuliano Silva Daniel P de Almeida e Silva João Batista de Almeida Lima Urgel |
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Affiliation: | (1) Department of Biotechnology, Faculty of Chemical Engineering of Lorena, Lorena-SP, 12600-970, Brazil;(2) Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Science, University of São Paulo, São Paulo-SP, Brazil |
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Abstract: | A 23 full factorial design was used to study the influence of different experimental variables, namely wort gravity, fermentation temperature and nutrient supplementation, on ethanol productivity from high gravity wort fermentation by Saccharomyces cerevisiae (lager strain), under pilot plant conditions. The highest ethanol productivity (0.69 g l–1 h–1) was obtained at 20°P [°P is the weight of extract (sugar) equivalent to the weight of sucrose in a 100 g solution at 20°C], 15°C, with the addition of 0.8% (w/v) yeast extract, 24 mg l–1 ergosterol and 0.24% (v/v) Tween 80. |
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Keywords: | ethanol factorial design fermentation high gravity brewing Saccharomyces cerevisiae |
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