胡萝卜培养细胞胚胎发生能力的差异

异质的培养细胞是植物细胞变异的一个重要来源。由Bergman建立的平板培养技术已在许多方面得到应用,其中特别有意义的是获得次生物质含量不同的细胞系和具有较好农艺性状的栽培植物品系。胡萝卜培养细胞是研究植物体细胞胚胎发

THE DIFFERENCE OF EMBRYOGENETIC ABILITY IN CULTURED CARROT CELLS

Carrot suspension cells were culturedby single cell technique and found thatgrowth and the content of pigment,espe-cially,the ability of embryogenesis ofsingle cell clones were great different.Under the light and on the basic MS me-dium where carrot callus could undergoembryogenesis,about half of clones ex-pressed differentiation,however;amongthem,half had a normal morphology,14% abnormal,19% roots only,18%embryos only.But,in the dark,majori-ty of clones could not express differen-tiation,34% could.Among them,46%formed roots,6% had a normal morpho-logy,28% abnormal.Callus derived fromsingle cell clones could not differentiateand could not still express differentiationon the medium of different combinationof auxin,cytokinin,gibberellic acid andabscisic acid.It's suggested that theylost morphogical ability in heridity. Different staining density and zymo-graphy of isoperoxidase were found incomparison of callus and differentiatedtissue,however;both types of tissue de-rived from single cell clones expressedsimilar difference also.