Hydrolysis of xylans by a thermostable hybrid xylanase expressed in <Emphasis Type="Italic">Escherichia coli</Emphasis> |
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Authors: | X Y Weng J Y Sun |
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Institution: | 1.Department of Biological Science, College of Life Science,Zhejiang University,Hangzhou,P. R. China;2.Microbiology Division of Feed Science Institute, College of Animal Science,Zhejiang University,Hangzhou,P. R. China |
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Abstract: | Escherichia coli-expressed a hybrid xylanase, Btx, encoded by a designed hybrid xylanase gene btx was purified. The molecular mass of the enzyme was estimated to be 22 kDa. The K
m and k
cat values for Btx were 1.9 mg/ml and 140 s−1, respectively. It hydrolyzed xylan principally to xylobiose and xylotriose, and was functionally similar to family 11 xylanases.
As some differences were found in the hydrolytic products between birchwood xylan and wheat bran insoluble xylan, the xylan
binding domains in xylanase Btx must have different effects on soluble and insoluble xylan. |
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Keywords: | |
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