The Polyclonal Antibodies Induced by VBP3 Complex Peptide Targeting Angiogenesis and Tumor Suppression |
| |
Authors: | Yanrui Deng Hui Liang Lei Pan Ruiqiang Weng Ligang Zhang Zhitao Wan Xuejun Lao Qing Zhang Likuan Xiong Ning Deng |
| |
Institution: | 1.Guangdong Province key Laboratory of Molecular Immunology and Antibody Engineering,College of Bioscience and Technology School in Jinan University,Guangzhou,China;2.Shenzhen Key Laboratory of Birth Defects in Baoan Maternal and Child Health Care,Affiliated Hospital in Jinan University,Shenzhen,China;3.Dept. of Gastrointestinal Surgery,the First Clinical School in Jinan University,Guangzhou,China;4.The State Key Laboratory of Biocontrol, School of Life Sciences,Sun Yat-Sen University,Guangzhou,China |
| |
Abstract: | Basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) play a critical role in tumor-associated angiogenesis and have become the targets of anti-tumor therapy. The BALB/c mice were immunized with VEGF/bFGF complex peptide (VBP3) constructed with different epitope peptides of human VEGF and bFGF. The results of the immunogenicity showed that the VBP3 could effectively stimulate immune response in mice and elicit the mice to produce high titer specific anti-VEGF and anti-bFGF antibodies (anti-VBP3 antibodies). The polyclonal anti-VBP3 antibodies separated from the mouse immune serum could effectively inhibit the proliferation, migration and tube formation of human umbilical vein endothelial cells (HUVECs) and block the proliferation and migration of lung cancer A549 cells. Besides, the anti-VBP3 antibodies could effectively inhibit tumor growth and tumor angiogenesis in BABL/c nude mice. The results demonstrated that the VBP3 complex peptide could elicit the body to produce the high titer anti-VEGF and anti-bFGF antibodies, which showed anti-tumor and anti-angiogenic effects in vitro and in vivo. The results revealed that the VBP3 complex peptide could be used as a potential peptide vaccine in tumor therapy. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|