Tissue-specific signal(s) activate the promoter of a metallocarboxypeptidase inhibitor gene family in potato tuber and berry |
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Authors: | Molnár Attila Lovas Ágnes Bánfalvi Zsófia Lakatos Lóránt Polgár Zsolt Horváth Sándor |
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Institution: | (1) Agricultural Biotechnology Center, P.O. Box 411, 2101 Gödöllö, Hungary;(2) Pannon Agricultural University, Festetics Gy. street 7, 8360 Keszthely, Hungary |
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Abstract: | The molecular basis of the differential expression of the GM7-type metallocarboxypeptidase inhibitor (MCPI) genes in tuberizing (StMCPI) and non-tuberizing Solanum species (SbMCPI) was investigated. It was shown that the StMCPI is encoded by a gene family in Solanum tuberosum (potato), but SbMCPI might be a single-copy gene in the non-tuberizing species Solanum brevidens. The StMCPI promoter shows evolutionary relatedness to the S. brevidens-derived SbMCPI and to the fruit-specific tomato promoter 2A11. Both StMCPI and SbMCPI promoter regions were able to confer tuber- and berry-specific expression for the -glucuronidase reporter gene in potato suggesting that the difference in MCPI gene expression is in trans regulatory factors between the tuberizing and the non-tuberizing Solanum species. The MCPI promoters did not respond to metabolic, environmental or hormonal signals in leaves. Thus, the MCPI genes are regulated in a different way than the other known tuber-specific genes and potentially are suitable for biotechnological application in potato to provide specific transgene expression in tuber and berry. |
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Keywords: | berry metallocarboxypeptidase inhibitor organ-specific gene expression potato tomato tuber |
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