Electron microscopy and restriction enzyme mapping reveal additional intervening sequences in the chicken ovalbumin split gene |
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Authors: | A.C. Garapin B. Cami W. Roskam P. Kourilsky J.P. Le Pennec F. Perrin P. Gerlinger M. Cochet P. Chambon |
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Affiliation: | Groupe de Biologie Moléculaire du Gène ERA, CNRS 201 Institut Pasteur Paris, France;Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS et U 44 de I''INSERM Faculté de Médecine Strasbourg, France |
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Abstract: | The Eco RI fragment “b” of chicken DNA (Breathnach, Mandel and Chambon, 1977), which contains the sequences coding for the 5′ quarter of ovalbumin mRNA (ov mRNA), has been isolated by molecular cloning using a “shotgun” approach. Electron microscopy and restriction enzyme analysis have revealed that the sequences coding for the 5′ quarter (~500 nucleotides) of ov mRNA are split into four regions separated by three intervening sequences. The cloning procedure seems to be reliable, since the restriction enzyme pattern of the cloned Eco RI fragment “b” is similar to that of the corresponding chromosomal DNA fragment. There is no evidence supporting the existence of a 150–200 nucleotide long sequence at the 5′ end of the ov mRNA similar to the “leader” sequences found at the 5′ end of some adenovirus and SV40 mRNAs. |
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