Transfer of cultured bovine embryos |
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Authors: | D.F. Peters G.B. Anderson R. BonDurant P.T. Cupps M. Drost |
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Affiliation: | 1. Department of Animal Science, University of California, Davis, CA 95616 USA;2. Department of Reproduction, School of Veterinary Medicine, University of California, Davis, CA 95616 USA;3. Present address; Department of Reproduction, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610 USA |
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Abstract: | A total of 126 bovine embryos were surgically collected from 16 superovulated donor heifers 5 days after estrus and randomly selected for either immediate transfer to synchronized recipients or culture at 37°C for 24 hours and subsequent transfer. Twenty-four of 56 (42.8%) embryos maintained for 24 hours in Ham's F10 medium supplemented with 10% heat treated fetal calf serum (HTFCS) and transferred to 32 recipients produced live calves. Survival of 70 noncultured embryos transferred to 35 recipients was 55.7% (39 calves). The percentages of recipients that were diagnosed pregnant at 42 days with cultured and control embryos were 59.4% () and 74.3% (), respectively. No statistical difference was observed between the cultured and control embryos for viability following transfer to recipient females.In a second study, Day 7 embryos maintained in Ham's F10 medium supplemented with 10% HTFC serum for various culture periods were tested for viability following nonsurgical transfer to recipient females. A total of , and embryos cultured for 24, 48 and 72 hours, respectively, resulted in pregnant recipients following transfer. |
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