Neutralization of cobra venom by cocktail antiserum against venom proteins of cobra (Naja naja naja) |
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| Affiliation: | 1. Depto. de Física e Biofísica, Instituto de Biociências, Universidade Estadual Paulista—UNESP, Botucatu, SP, Brazil;2. Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José 11501, Costa Rica;1. Department of Studies in Biochemistry, University of Mysore, Manasagangothri, Mysore 570006, Karnataka, India;2. Department of Studies in Biochemistry, Tumkur University, Tumkur, Karnataka, India;1. National Tsing Hua University, College of Life Sciences, Hsinchu 300, Taiwan;2. National Health Research Institutes, National Institute of Infectious Diseases and Vaccinology, Zhunan, Miaoli 350, Taiwan;3. Chang Gung University, Department of Biochemistry and Molecular Biology, Tao-Yuan 333, Taiwan;4. Mithra Biotechnology Inc., New Taipei City 221, Taiwan;1. Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;2. Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia |
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| Abstract: | Naja naja venom was characterized by its immunochemical properties and electrophoretic pattern which revealed eight protein bands (14 kDa, 24 kDa, 29 kDa, 45 kDa, 48 kDa, 65 kDa, 72 kDa and 99 kDa) by SDS-PAGE in reducing condition after staining with Coomassie Brilliant Blue. The results showed that Naja venom presented high lethal activity. Whole venom antiserum or individual venom protein antiserum (14 kDa, 29 kDa, 65 kDa, 72 kDa and 99 kDa) of venom could recognize N. naja venom by Western blotting and ELISA, and N. naja venom presented antibody titer when assayed by ELISA. The neutralization tests showed that the polyvalent antiserum neutralized lethal activities by both in vivo and in vitro studies using mice and Vero cells. The antiserum could neutralize the lethal activities in in-vivo and antivenom administered after injection of cobra venom through intraperitoneal route in mice. The cocktail antiserum also could neutralize the cytotoxic activities in Vero cell line by MTT and Neutral red assays. The results of the present study suggest that cocktail antiserum neutralizes the lethal activities in both in vitro and in vivo models using the antiserum against cobra venom and its individual venom proteins serum produced in rabbits. |
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| Keywords: | ELISA MTT Neutral red Cocktail antiserum Cobra |
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