Type V collagen counteracts osteo-differentiation of human mesenchymal stem cells |
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| Affiliation: | 1. Department of Biomedical Engineering, University of Connecticut, Storrs, 263 Farmington Ave, Farmington, CT 06030, CT, United States;2. New England Musculoskeletal Institute, UConn Health, 263 Farmington Ave, Farmington, CT 06030, CT, United States;3. Department of Medicine, UConn Health, 263 Farmington Ave, Farmington, CT 06030, CT, United States |
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| Abstract: | In search of novel gene signatures for osteo-differentiation of mesenchymal stem cells (MSCs), we submitted cDNA preparations from undifferentiated and differentiating MSCs to differential display- and semiquantitative-PCR and found down-regulation of COL5A1 in osteo-induced cultures at days 21 and 28, when the mineralized matrix accumulates. We also cultured osteo-differentiating MSCs onto type V collagen substrates and found a decrease in the accumulation of extracellular calcium compared to those grown in uncoated flasks. To our knowledge, this is first evidence that type V collagen might represent a stromal component that impairs osteogenesis. |
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| Keywords: | Stem cells Collagen Osteogenesis Gene expression MSC" },{" #name" :" keyword" ," $" :{" id" :" kwrd0035" }," $$" :[{" #name" :" text" ," _" :" mesenchymal stem cell BM" },{" #name" :" keyword" ," $" :{" id" :" kwrd0045" }," $$" :[{" #name" :" text" ," _" :" bone marrow PCR" },{" #name" :" keyword" ," $" :{" id" :" kwrd0055" }," $$" :[{" #name" :" text" ," _" :" polymerase chain reaction dNTPs" },{" #name" :" keyword" ," $" :{" id" :" kwrd0065" }," $$" :[{" #name" :" text" ," _" :" deoxynucleotide triphosphates DD" },{" #name" :" keyword" ," $" :{" id" :" kwrd0075" }," $$" :[{" #name" :" text" ," _" :" differential display |
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