A novel monoclonal antibody to Neisseria meningitidis serogroup X capsular polysaccharide and its potential use in quantitation of meningococcal vaccines |
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| Affiliation: | 1. Research and Development Vicepresidency, Finlay Institute, La Habana, A.P. 16017, Cod. 11600, Cuba;2. Department of Pharmacology, Faculty of Biological Sciences, University of Concepción, Concepción, P.O. Box 160C, Chile;1. Division for Virology, Centre for Infectious Diseases Control, National Institute for Public Health and the Environment (RIVM), P.O. Box 1, 3720 BA Bilthoven, The Netherlands;2. Laboratory of Clinical and Epidemiological Virology, Department of Microbiology and Immunology, Rega Institute for Medical Research, University of Leuven, Leuven, Belgium;3. Virology Department, Erasmus Medical Center, Rotterdam, The Netherlands;1. Grant Medical Center, 323 East Town Street, First Floor, Suite 100, Columbus, OH 43215, USA;2. Division of Surgery and Biomechanics, Kent State University College of Podiatric Medicine, 6000 Rockside Woods Boulevard, Independence, OH 44131, USA;3. Kent State University College of Podiatric Medicine, 6000 Rockside Woods Boulevard, Independence, OH 44131, USA;1. Center for Cell Transplantation (Seventh Unit of General Surgery Department), Institute of Organ Transplantation, Sichuan Academy of Medical Sciences & Sichuan Provincial People''s Hospital, Chengdu 610072, China;2. Department of Blood Medicine, Sichuan Academy of Medical Sciences & Sichuan Provincial People''s Hospital, Chengdu 610072, China;3. Department of Radiology, Sichuan Academy of Medical Sciences & Sichuan Provincial People''s Hospital, Chengdu 610072, China;1. Public Health England, Public Health Laboratory, Manchester, Manchester Medical Microbiology Partnership, PO Box 209, Clinical Sciences Building II, Manchester Royal Infirmary, Manchester M13 9WZ, UK;2. Immunisation Department, Health Protection Services, Public Health England, Colindale, London NW9 5EQ, UK;3. University of Manchester, Inflammation Sciences Research Group, School of Translational Medicine, Stopford Building, Manchester M13 9PL, UK |
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| Abstract: | A novel murine hybridoma monoclonal antibody (MAb) was produced against the capsular polysaccharide (CP) of Neisseria meningitidis serogroup X (MenX) in order to develop a sandwich enzyme linked immunosorbent assay (ELISA) for the quantitation of the meningococcal polysaccharide. The MAb only reacted with the CP from MenX and did not react with CPs from N. meningitidis serogroups A, C, Y and W (MenA, MenC, MenY, MenW). The affinity constant (Ka) of the MAb measured by non-competitive ELISA was 7.25 × 107 M−1. The application of this MAb in a sandwich ELISA was demonstrated by its ability to properly quantitate three lots of an experimental meningococcal CP-based vaccine. The MAb obtained in this work could be a valuable reagent for the detection and quantitation of future meningococcal vaccines containing MenX CP. |
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| Keywords: | Monoclonal antibody ELISA Quantitation |
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