Chicken anemia virus and avian gyrovirus 2 as contaminants in poultry vaccines |
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| Institution: | 1. FEPAGRO – Saúde Animal, Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Estrada do Conde 6000, Eldorado do Sul CEP 92990-000, Rio Grande do Sul, Brazil;2. Laboratório de Virologia, Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde, UFRGS. Av. Sarmento Leite 500, Sala 208, Porto Alegre CEP 90050-170, Rio Grande do Sul, Brazil;3. EMBRAPA Suínos e Aves, BR 153 Km 110, Concordia CEP: 89700-000, SC, Brazil;1. Department of Poultry Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt;2. Department of Pathology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt;3. Department of Pharmacology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt;1. Immunology Section, Indian Veterinary Research Institute (IVRI), Izatnagar, Uttar Pradesh 243122, India;2. Avian Diseases Section Indian Veterinary Research Institute (IVRI) , Izatnagar, Uttar Pradesh 243122, India;3. Division of Veterinary Public Health, Indian Veterinary Research Institute (IVRI) , Izatnagar, Uttar Pradesh 243122, India;4. Department of Veterinary Microbiology, College of Veterinary Sciences, Pandit Deen Dayal Upadhaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura, Uttar Pradesh, 281001, India;5. Indian Veterinary Research Institute (IVRI), Izatnagar, Uttar Pradesh 243122, India;1. College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, Heilongjiang, PR China;2. Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Harbin 150030, Heilongjiang, PR China;3. Food College, Northeast Agricultural University, Harbin 150030, Heilongjiang, PR China;4. Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shanxi, PR China |
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| Abstract: | This study focuses on the detection of chicken anemia virus (CAV) and avian gyrovirus 2 (AGV2) genomes in commercially available poultry vaccines. A duplex quantitative real-time PCR (dqPCR), capable of identifying genomes of both viruses in a single assay, was employed to determine the viral loads of these agents in commercially available vaccines. Thirty five vaccines from eight manufacturers (32 prepared with live and 3 with inactivated microorganisms) were examined. Genomes of CAV were detected as contaminants in 6/32 live vaccines and in 1/3 inactivated vaccines. The CAV genome loads ranged from 6.4 to 173.4 per 50 ng of vaccine DNA (equivalent to 0.07 to 0.69 genome copies per dose of vaccine). Likewise, AGV2 genomes were detected in 9/32 live vaccines, with viral loads ranging from 93 to 156,187 per 50 ng of vaccine DNA (equivalent to 0.28–9176 genome copies per dose of vaccine). These findings provide evidence for the possibility of contamination of poultry vaccines with CAV and AGV2 and they also emphasize the need of searching for these agents in vaccines in order to ensure the absence of such potential contaminants. |
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| Keywords: | Chicken anemia virus Avian gyrovirus 2 Vaccine contaminants |
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