Ultrasonic Nanobubbles Carrying Anti-PSMA Nanobody: Construction and Application in Prostate Cancer-Targeted Imaging |
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| Authors: | Xiaozhou Fan Luofu Wang Yanli Guo Zhui Tu Lang Li Haipeng Tong Yang Xu Rui Li Kejing Fang |
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| Affiliation: | 1. Department of Ultrasound, Southwest Hospital, Third Military Medical University, Chongqing, China.; 2. Department of Urology, Daping Hospital, Institute of Surgery Research, Third Military Medical University, Chongqing, China.; 3. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang City, Jiangxi Province, China.; 4. Jiangxi-QAI Joint Research Institute, Nanchang University, Nanchang City, Jiangxi Province, China.; Vrije Universiteit Brussel, BELGIUM, |
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| Abstract: | To facilitate prostate cancer imaging using targeted molecules, we constructed ultrasonic nanobubbles coupled with specific anti-PSMA (prostate specific membrane antigen) nanobodies, and evaluated their in vitro binding capacity and in vivo imaging efficacy. The “targeted” nanobubbles, which were constructed via a biotin-streptavidin system, had an average diameter of 487.60 ± 33.55 nm and carried the anti-PSMA nanobody as demonstrated by immunofluorescence. Microscopy revealed targeted binding of nanobubbles in vitro to PSMA-positive cells. Additionally, ultrasonography indicators of nanobubble imaging (including arrival time, peak time, peak intensity and enhanced duration) were evaluated for the ultrasound imaging in three kinds of animal xenografts (LNCaP, C4-2 and MKN45), and showed that these four indicators of targeted nanobubbles exhibited significant differences from blank nanobubbles. Therefore, this study not only presents a novel approach to target prostate cancer ultrasonography, but also provides the basis and methods for constructing small-sized and high-efficient targeted ultrasound nanobubbles. |
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