A radioimmunoassay for the Gm(b0) allotype of human immunoglobulins |
| |
| Authors: | Howard M Reisner Peter B Imrey William J Yount |
| |
| Institution: | (1) Department of Pathology, Bacteriology and Immunology, University of North Carolina at Chapel Hill, 27514, North Carolina;(2) Department of Pathology and Medicine, University of North Carolina at Chapel Hill, 27514, North Carolina;(3) Department of Pathology, School of Medicine and Department of Biostatistics, University of North Carolina at Chapel Hill, 27514, North Carolina;(4) Department of Pathology, School of Public Health, University of North Carolina at Chapel Hill, 27514, North Carolina;(5) School of Basic Medical Sciences at Urbana Champaign, University of Illinois, 61801 Urbana, Illinois |
| |
| Abstract: | A radioimmunoassay for the human allotype Gm(b0) which provides a sensitive and quantitative measurement of the level of this IgG3 genetic marker has been developed. The assay system can detect 15 nanograms of Gm(b0) IgG3 protein and is not inhibited by immunoglobulins of other allotypes and isotypes. Using this assay, good correlation was found between IgG3 and Gm(b0) levels in homozygous Gm(f, b0) sera and gene dosage effects could be confirmed. The correlation between Gm(b0) levels and IgG3 in Negroid Gm(a, b0) sera was not as good. This reduced correlation has been attributed to antigen differences in the IgG3 Gm markers characteristic of some Negroid Gm(a, b0) sera. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
