Enzymatic activity and filament assembly of Acanthamoeba myosin II are regulated by adjacent domains at the end of the tail |
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Authors: | M A Atkinson E Appella M A Corigliano-Murphy E D Korn |
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Institution: | Laboratory of Cell Biology, National Heart, Lung and Blood Institute, Bethesda, MD 20892. |
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Abstract: | Polyclonal antibodies raised against a synthetic peptide consisting of the last 19 amino acids at the end of the coiled-coil region of the heavy chains inhibited the actin-activated Mg2+-ATPase activity of myosin II and its ability to form filaments. Antibodies against a synthetic peptide corresponding to the 21 adjacent amino acids at the beginning of the non-helical tailpiece, which include the three regulatory phosphorylatable serines, had no effect on either activity. |
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