Restriction fragment length polymorphism (RFLP) analysis of bovine nuclear protein genes |
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Authors: | S A Krawetz R A Bricker W Connor R B Church G H Dixon |
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Institution: | (1) Department of Medical Biochemistry, Faculty of Medicine, University of Calgary, 3330 Hospital Drive N.W., T2N 4N1 Calgary, Alberta, Canada |
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Abstract: | Summary We have recently cloned both the bovine protamine (Krawetz et al. 1987, DNA 6: 47–57) and high mobility group (HMG-1) cDNAs (Pentecost and Dixon 1984, Bioscience Reports 4: 49–57). They have been used as probes for Restriction Fragment Length Polymorphism analysis of male-female pairs of different species and breeds, within the genus Bos. Utilizing this approach we have studied inheritance, chromosomal location and gene copy number of the bovine protamine and HMG-1 genes. This revealed that these nuclear protein genes are highly conserved suggesting that selective pressure has maintained their gene structures during evolution. A polymorphic Taq 1 restriction fragment was identified that was shown to be a heritable marker. These genes are not sex-linked and are present in a single copy for protamine and at least two copies for the HMG-1. |
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Keywords: | RFLP (restriction fragment length polymorphism) Protamine HMG-1 (high mobility group-1)-Hybridization |
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