Investigations of mannan and glucan in the cell wall ofCandida boidinii cultivated on methanol |
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| Authors: | T. Hristozova L. Michailova V. Dmitriev A. Tsiomenko Z. Roshkova D. Tuneva |
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| Affiliation: | (1) Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria;(2) Institute of Biochemistry and Physiology of Microorganisms, Academy of Sciences, Pushchino, Moskow region, Russia |
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| Abstract: | The polysaccharide components (mannan and glucan) in the cell wall ofCandida boidinii M 363 grown on methanol and glucose as control were investigated using electron microscopy, cytochemical and biochemical methods. An ultrastructural rearrangement of the polymers in the cell wall of yeasts cultivated on methanol in comparison to those cultivated on glucose was established. The morphological changes correlate to the quantitative changes in the polysaccharide constituents of the cell wall. The forming and the role of thiosemihydrocarbazide (TSHC) — negative zones in theCandida boidinii cell wall cultivated on methanol media are discussed. |
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| Keywords: | cell wall mannan glucan Candida boidinii ultrastructure methylotrophic yeasts |
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