Chemical Characterization of the Callose Plug Isolated from Camellia japonica Pollen Tube |
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| Authors: | Nakamura, Norio Mori, Masao Suzuki, Hiroshi |
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| Affiliation: | 1Department of Biology, Yokohama City University Kanazawa-ku, Yokohama 236, Japan
2Institute of Biological Sciences, University of Tsukuba Sakura-mura, Ibaraki 305, Japan |
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| Abstract: | A partially purified preparation of callose plug was obtainedfrom Camellia japonica pollen tube by limited enzymolysis anddensity centrifugation. The plug preparation consisted of proteinand polysaccharide at about 24% and 58% of dry weight, respectively.The plug polysaccharide was demonstrated to be a rß-1,3-glucanby staining reaction, acid hydrolysis, enzymatic digestion,infrared spectroscopy and methylation analysis. The averagedegree of polymerization of this glucan was calculated to beat least 90. This indicates that the plug callose has a longerchain than the tube wall callose. (Received June 15, 1983; Accepted November 26, 1983) |
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