|
|||||
|
|
Isolation of phospholipase d producing microorganisms with high transphosphatidylation activity |
|
| Authors: | Tairo Hagishita Masanobu Nishikawa Tadashi Hatanaka |
| Institution: | (1) Research Institute for Biological Sciences, Okayama (RIBS), 7549-1, Kayo-cho, Jyobo-gun, Okayama , 716-1241, Japan;(2) Present address: Tokyo Metropolitan Institute of Gerontology, Sakae-machi, Itabashi-ku, Tokyo , 173-0015, Japan |
| Abstract: | The transphosphatidylation and hydrolytic activities of phospholipase d in culture supernatants of soil isolates were evaluated by a specific spectrophotometric method for quantitative determination using an artifical substrate, phosphatidyl-p-nitrophenol. Phospholipase d from strain TH-2 showed the highest specific activity and ratio of transphosphatidylation activity to hydrolytic activity among those from the eight soil isolates and commercial Actinomycetes phospholipase d. |
| Keywords: | Actinomycetes phospholipase d phospholipid transphosphatidylation |
| 本文献已被 SpringerLink 等数据库收录！ | |