Low cytotoxicity effect of dendrosome as an efficient carrier for rotavirus VP2 gene transferring into a human lung cell line |
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Authors: | Farzaneh Pourasgari Shahin Ahmadian Ali Hatef Salmanian Mohammad Nabi Sarbolouki Mohammad Massumi |
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Affiliation: | (1) Institute of Biochemistry and Biophysics, University of Tehran, Enghelab avenue, P.O. Box: 13145-1384, Tehran, Iran;(2) National Institute for Genetic Engineering and Biotechnology, Pajoohesh Blvd., Tehran-Karaj Highway, 17th Km., P.O. Box: 14155-6343, Tehran, Iran;(3) Present address: Research Unit on Cellular and Molecular Biology, Institute Municipal d’Investigació Mèdica (IMIM), University of Pompeu Fabra, Dr. Aiguader, 80, Barcelona, 08003, Spain |
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Abstract: | The efficiency of dendrosome (a gene porter) was assessed in transferring recombinant human rotavirus VP2 cDNA into A549, a human lung cell line. After gene transferring, transmission electron microscopy showed core-like particles (CLPs) formation in the transfected cells both with dendrosome and lipofectamine porters. In addition, western blotting analysis showed that the expression of VP2 gene was almost equal in the dendrosome and lipofectamine-transfected cells. Also, the cytotoxicity studies revealed that dendrosome had a lower cytotoxicity than lipofectamine. Therefore, our study may introduce dendrosome as a possible carrier for gene transferring into the human lung cell line, especially, for intranasally administration of DNA vaccines. |
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Keywords: | Dendrosome Lipofectamine DNA Vaccine VP2 A549 VLP |
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