Role of CheY1 and CheY2 in the Chemotaxis of A. tumefaciens Toward Acetosyringone |
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Authors: | Behrouz Harighi |
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Affiliation: | (1) Department of Plant Protection, College of Agriculture & Natural Resources, University of Kurdistan, Sanandaj, Iran |
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Abstract: | Agrobacterium tumefaciens has a chemtaxis operon, which includes orf1, orf2, cheY1, cheA, cheR, cheB, cheY2, orf9, and orf10. In-frame deletions of cheY1 and cheY2 were constructed and the chemosensory behavior of the mutants was examined on swarm plates and in a chemotaxis assay toward acetosyringone. The cheY2 mutant (C1/delY2) showed impaired chemotactic capabilities in both swarming and chemotaxis assays. The effect of lacking CheY1 on chemotaxis is less severe than that of CheY2, under the conditions studied. |
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