Optimization of pulsed field gel electrophoresis (PFGE) conditions for thermophilic bacilli |
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| Authors: | Elif Yavuz Hatice Gunes Sebnem Harsa Cisem Bulut A. Fazil yenidunya |
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| Affiliation: | (1) Biotechnology and Bioengineering Programme, Izmir Institute of Technology, Izmir, Turkey;(2) Department of Biology, Science Faculty, Izmir Institute of Technology, Izmir, Turkey |
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| Abstract: | Although thermophilic members of the genus Bacillus are known to tolerate extreme environmental conditions, they appeared to be readily damaged upon mechanical manipulations. This fact was evidenced in genotyping of some strains of thermophilic Bacillus by pulsed field gel electrophoresis (PFGE). Consequently, a new procedure for the preparation of agarose plugs was developed. The procedure produced interpretable genomic DNA restriction profiles. |
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| Keywords: | DNA degradation genotyping pulsed field gel electrophoresis thermophilic Bacillus |
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