Cell cycle analysis with bromodeoxyuridine pulselabeling in asynchronous hybridoma cultures |
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| Authors: | Lance S Williams Richard A Richieri Judith A Nordberg Pao C Chau |
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| Institution: | (1) Biology Department, University of California, 92093-0310 San Diego, CA;(2) Flow Cytometry Lab, VA Medical Center University of California, 92093-0310 San Diego, CA;(3) Department of AMES/Chemical Engineering, University of California, 92093-0310 San Diego, CA |
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| Abstract: | Summary The cell cycle kinetics of a mouse hybridoma was examined by immunocytochemical staining of incorporated bromodeoxyuridine in an asynchronous culture. The cell cycle phase traverse times were extracted from a time series of bivariate distributions of incorporated bromodeoxyuridine and total DNA content; the G1, S and G2/M phase traverse times were 7, 9 and 4 h, respectively in the exponential growth phase. |
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