Evaluation of phytomedicinal yield potential and molecular profiling of micropropagated and conventionally grown turmeric (<Emphasis Type="Italic">Curcuma longa</Emphasis> L.) |
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Authors: | Shikha Singh Ananya Kuanar Sujata Mohanty Enketeswara Subudhi Sanghamitra Nayak |
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Institution: | (1) Centre of Biotechnology, Bhubaneswar, Orissa, India; |
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Abstract: | Drug yielding potential of turmeric (Curcuma longa L.) is due to the presence of important phytoconstituents such as curcumin, oleoresin and essential oil. Slow multiplication
rate, high susceptibility to rhizome rot and leaf spot disease and restricted availability of elite genotype necessitated
application of tissue culture technique to alleviate the problems. A protocol has been developed for in vitro micropropagation
of an elite genotype (cv. suroma) using latent axillary bud explants from unsprouted rhizome, available throughout the year.
MS media containing 3 mg/l 6-Benzyladenine (BA) and 1 mg/l Indole Acetic acid (IAA) was found optimum for regeneration, multiplication
and in vitro conservation of plantlets. After 3 years of in vitro conservation regenerants were transplanted to field and
assessed for drug yielding potential through evaluation of curcumin, oleoresin and essential oil contents of rhizomes and
leaves. One year of field grown tissue culture derived turmeric were found uniform in all the characteristics examined, when
compared with those grown conventionally. Micropropagated turmeric showing stable drug yielding potential also proved to have
genetic basis of stability as revealed by RAPD based molecular profiling. |
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