Shoot organogenesis and somatic embryogenesis from leaf and shoot explants of <Emphasis Type="Italic">Ochna integerrima</Emphasis> (Lour) |
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Authors: | Guohua Ma Jinfeng Lü Jaime A Teixeira da Silva Xinhua Zhang Jietang Zhao |
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Institution: | (1) Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, The Chinese Academy of Sciences, 510650 Guangzhou, China;(2) Graduate University of Chinese Academy of Sciences, 100039 Beijing, China;(3) Faculty of Agriculture and Graduate School of Agriculture, Kagawa University, Miki-cho Ikenobe 2393, Kagawa-ken 761-0795, Japan |
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Abstract: | Ochna integerrima is a medicinal and ornamental plant in Southeastern Asia. It has been listed as a rare and endangered species in China. Here
we studied the effects of plant growth regulators and their concentrations on the induction of somatic embryogenesis and shoot
organogenesis from leaf and shoot explants of O. integerrima for the first time. Cytokinins played a crucial role in somatic embryogenesis and shoot organogenesis. Among them, a higher
concentration of thidiazuron (10.0–15.0 μM TDZ) could induce both somatic embryogenesis and adventitious shoot formation whereas
low concentrations of TDZ (5.0 μM) could only induce adventitious shoots. However, 6-benzyladenine (BA at 5–15 μM) could only
induce adventitious shoots. Shoot explants induced more adventitious shoots and somatic embryos than leaf explants when cultured
on medium with the same concentration (5–15 μM) of TDZ or 15 μM BA. Medium containing 0.5 μM α-naphthaleneacetic acid and
8 μM indole-3-butyric acid and 0.1% activated charcoal could induce adventitious roots within 1 month. An efficient mass propagation
and regeneration system has been established. |
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Keywords: | |
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