Langmuir-Blodgett monolayer films of the Rhodopseudomonas viridis reaction center: determination of the order of the hemes in the cytochrome c subunit. |
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Authors: | G Alegria P L Dutton |
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Institution: | Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia 19104. |
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Abstract: | The Langmuir-Blodgett (LB) film technique has been applied to produce oriented and photo-active films of isolated reaction center cytochrome c complexes (RC-cyt c) and chromatophore membranes from the photosynthetic bacterium Rhodopseudomonas viridis. Linear dichroism (LD) and redox potentiometry have been used to identify the four cytochrome c hemes of the RC-cyt c complex. Resolved angular orientations of the four hemes in LB films of both isolated RC-cyt-c complexes and of chromatophore membranes permit the reduction of the 24 possible arrangements to two. Additional structural and functional information from other sources allows us to propose a model which best accounts for all the available experimental data. |
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