Characterization and regulation of PiDur3, a permease involved in the acquisition of urea by the ectomycorrhizal fungus Paxillus involutus |
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Authors: | Morel Mélanie Jacob Christophe Fitz Michael Wipf Daniel Chalot Michel Brun Annick |
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Affiliation: | Faculty of Sciences and Technology, Research Unit INRA/UHP 1136 Tree-microbe Interactions, Nancy-University, BP 239, F54506 Vandoeuvre-les-Nancy Cedex, France. Melanie.Morel@scbiol.uhp-nancy.fr |
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Abstract: | Urea, which is known to be a source of nitrogen for the growth of many organisms, represents an important fertilizer in forest soils. Since most trees form symbiotic associations with ectomycorrhizal fungi, the capacities of these symbionts to take up and assimilate urea would determine the efficiency of urea nitrogen salvaging by plants. We showed that Paxillusinvolutus, an ectomycorrhizal basidiomycete, is capable of using urea as sole nitrogen source. We report the molecular characterization of an active urea transporter (PiDur3) isolated from this fungus. We demonstrated that the import of urea is a minor event on ammonium condition, since the expression of PiDUR3 is repressed by the high intracellular glutamine pool. Interestingly, on urea nutritive condition, the uptake of urea is rather mediated by the intracellular urea pool and particularly by urease efficiency. |
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