A proton nuclear magnetic resonance study on the solution structure of crotamine |
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| Authors: | Toshiya Endo Masanao Oya Hiroshi Ozawa Yoshio Kawano José R Giglio and Tatsuo Miyazawa |
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| Institution: | (1) Department of Chemistry, College of Technology, Gunma University, Tenjin-cho, 376 Kiryu, Japan;(2) Computer Centre, University of Tokyo, Yayoi, Bunkyo-ku, 113 Tokyo, Japan;(3) Instituto de Química, Universidade de São Paulo, Caixa Postal 20780, CEP 01000 São Paulo, Brazil;(4) Departamento de Bioquímica, Faculdade de Medicina, Universidade de São Paulo, CEP 14049 Ribeirão Preto, São Paulo, Brazil;(5) Faculty of Engineering, Yokohama National University, Hodogaya-ku, 240 Yokohama, Japan |
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| Abstract: | Proton nuclear magnetic resonance (NMR) spectra of crotamine, a myotoxic protein from a Brazilian rattlesnake (Crotalus durissus terrificus), have been analyzed. All the aromatic proton resonances have been assigned to amino acid types, and those from Tyr-1, Phe-12, and Phe-25 to the individual residues. ThepH dependence of the chemical shifts of the aromatic proton resonances indicates that Tyr-1 and one of the two histidines (His-5 or His-10) are in close proximity. A conformational transition takes place at acidicpH, together with immobilization of Met-28 and His-5 or His-10. Two sets of proton resonances have been observed for He-17 and His-5 or His-10, which suggests the presence of two structural states for the crotamine molecule in solution. |
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| Keywords: | NMR crotamine myotoxin rattlesnake venom structural heterogeneity |
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