拟南芥雄性不育突变体ms1142的遗传定位与功能分析

经EMS诱变野生型拟南芥(Arabidopsis thaliana)群体筛选得到一株雄性不育突变体ms1142, 突变体的果荚短小, 不含种子。细胞学观察和扫描电镜结果表明, 突变体花药发育过程中, 花药中小孢子外壁异常、破裂, 最后没有花粉形成。遗传分析表明, 该突变体为隐性单核基因突变所致; 利用图位克隆的方法将MS1142基因定位于第1条染色体的BAC克隆F16P17上44 kb区间内, 目前尚未见该区间内有雄性不育基因的报道。以上结果结合生物信息学分析表明, MS1142是一个新的调控花药发育的关键基因。该工作为花药发育关键基因MS1142的克隆及功能分析奠定了基础。

Genetic Mapping and Functional Analysis of Arabidopsis thaliana Male Sterile Mutant ms1142

A male sterile mutant, ms1142, was isolated from a population of ethyl methane sulfonate (EMS)-treated wild-type Arabidopsis thaliana. The mutant has small siliques with no seed set. Cytological observation showed that during anther development, the microspores ruptured and no pollen grains formed in the mutant anther locules. Scanning electron microscopy revealed an aberrant and broken microspore exine in the mutant. Genetic analysis indicated that the mutant is controlled by a single recessive nuclear gene. Map-based cloning mapped the mutant gene MS1142 to a region of 44 kb in BAC F16P17 on chromosome 1. Bioinformatics analysis revealed no previous male sterile gene cloned in this region, so MS1142 is a new gene involved in anther and pollen development. These results set the basis for cloning and functional analysis of this gene controlling anther development.