Adentylate cyclase activity in myocardium of spontaneously hypertensive rat: effect of endogenous factors and solubilization |
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Authors: | R C Bhalla R V Sharma A T Ashley |
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Affiliation: | 1. Department of Anatomy University of Iowa, Iowa City, Iowa 52242 USA;2. Cardiovascular Center University of Iowa, Iowa City, Iowa 52242 USA |
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Abstract: | The isoproterenol- and glucagon-stimulated adenylate cyclase activities in the myocardial membranes of hypertensive rat were consistantly lower as compared with normal controls. Addition of cytosolic fraction (100,000 x supernatant) to the particulate preparation had an additive effect for glucagon and Gpp(NH)p stimulated enzyme activity and a synergistic effect for isoproterenol stimulation. Cytosolic fraction of normal control animals did not bring the adenylate cyclase activity in SHR equivalent to the control values. The basal and F?-stimulated enzyme activity of solubilized adenylate cyclase was reduced by about 30% in SHR as compared with WKY, which could be due to a decrease in the actual amount of adenylate cyclase in the myocardium of SHR. |
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Keywords: | SHR Spontaneously hypertensive rat WKY Normal Kyoto Wistar rat Gpp(NH)p guanylylimidodiphosphate PEP phosphoenolpyruvate cyclic AMP adenosine-3′,5′-monophosphate ATP adenosine 5′-triphosphate |
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