| 两种前S蛋白与HBV复制关系的初步研究 |
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| 引用本文： | 张永源,鄢璞,汪由坤,喻植群,郝连杰.两种前S蛋白与HBV复制关系的初步研究[J].Virologica Sinica,1990(4). |
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| 作者姓名： | 张永源 鄢璞 汪由坤 喻植群 郝连杰 |
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| 作者单位： | 同济医科大学同济医院临床免疫研究室，同济医科大学同济医院临床免疫研究室，同济医科大学同济医院临床免疫研究室，同济医科大学同济医院临床免疫研究室，同济医科大学同济医院临床免疫研究室 武汉 430030，武汉 430030，武汉 430030，武汉 430030，武汉 430030 |
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| 摘 要： | 乙型肝炎病毒(HBV)前S基因及其产物的发现有助于认识HBV复制规律。一些研究提示:前S_1蛋白是完整病毒颗粒表面的必要成分,其存在与HBV复制密切相关,前S_1检测能敏感和特异地反映HBV复制状态。另一些研究则认为:前S_2蛋白含有人多聚白蛋白的结合位点,并提出前S_2蛋白为HBV复制的新标志。为探讨HBV复制与两种前S蛋白之间的联系,本研究应用原位分子杂交分析一组慢性肝炎患者肝细胞内HBV DNA,同时检测肝细胞内HBsAg、HBcAg与两种前S蛋白,综合分析两种前S蛋白在HBV复制中的地位和作用。
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| 关 键 词： | 前S基因 前S蛋白 HBV复制 HBV标记 原位杂交 |
Comparison of Two Pre-s Proteins in Hepatocytes with HBV Replication Status by in situ HBV DNA Hybridization Assay |
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| Zhang Yon-yuan Yan Pu Wang You-kun Yu Ahi-qun Hao Lian-jie.Comparison of Two Pre-s Proteins in Hepatocytes with HBV Replication Status by in situ HBV DNA Hybridization Assay[J].中国病毒学(英文版),1990(4). |
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| Authors: | Zhang Yon-yuan Yan Pu Wang You-kun Yu Ahi-qun Hao Lian-jie |
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| Abstract: | To compare relationship between two pre-s proteins and HBV replication HBV DNA in situ hybridization was applied to a group of patients with chronic hepatitis by bio-probe, in conjunction of detection of intrahepatic HBsAg, HBcAg, pre-s1 and pre-s2 antigens. It was found that the positive rate for pre-s1 was up to 75%(15/20), being significantly higher than that of pre-s2 (35%) in the same group. More importantly the presence of pre..sl, rather than pre-s2, was in parallel with the presence of intrahepatic HBV DNA and HB_cAg. All findings suggest that pre-s1 was mainly expressed during HBV replication, indicating HBV replication. So detection of pre-s1 might substitute or complement HBe system and HBV DNA tests. |
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| Keywords: | Pre-s gene Pre-s proteins HBV replication HBV markers In situ hybridization |
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