改良DDRT-PCR研究苦参碱诱导K562细胞分化相关的差异表达基因

苦参碱是传统中药苦参的有效成分之一 .我们的前期实验表明 ,一定浓度的苦参碱可诱导人白血病K5 6 2细胞向成熟方向分化1] .为深入研究其诱导分化的分子机制 ,本文以改良的DDRT PCR技术分析苦参碱作用K5 6 2细胞前后基因表达的差异 ,并利用生物信息学的方法对差异表达的基因进行比较与分析 .1　材料与方法1 1　材料1 1 1　细胞株　对照组K5 6 2细胞与苦参碱 (0 2g L)处理组K5 6 2细胞 .1 1 2　E .coliJM10 9工程菌　引自重庆医科大学附二院肝炎研究所 .1 1 3　主要试剂　苦参碱 (Mr =2 4 8 36 ,纯度99 9% ) :由日本大正制药公司惠…

The Differentially Expressed Genes Related to Matrine-induced K562 Cell Differentiation by Improved DDRT-PCR

The differentiation of human leukemia K562 cells can be induced by matrine. To investigate the mechanism of the induced cell differentiation, an improved DDRT PCR technique was applied to screen the differentially expressed genes between K562 cells and the matrine treated cells. The sequences of the fragments of the differentially expressed genes were obtained by sequencing following the T A cloned fragments identified by restriction endonuclease, and analyzed with bioinformatics. The results indicated that the multiple gene expression known already changed in the process of induced cell differentiation and an unknown gene fragment was cloned. It suggested that the differentially expressed genes acted as cell differentiation related genes involved in the early phase of the induced cell differentiation.