Characterization of cyclic nucleotide phosphodiesterases in Xenopus laevis ovary |
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Authors: | C Allende M Plaza |
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Institution: | Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Santiago. |
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Abstract: | A cGMP-stimulated cyclic nucleotide phosphodiesterase present in cytosol of Xenopus laevis ovary has been purified and characterized. A cAMP-specific phosphodiesterase which is not activated by either cGMP or calmodulin, has also been characterized. Brief exposure of intact oocytes to 10 micro M progesterone results in an increase in activity of the cAMP-specific enzyme. The cGMP-stimulated and the calmodulin-activated phosphodiesterases are not altered. Changes in cyclic nucleotide levels during progesterone-induced maturation of oocytes may be modulated by these isoenzymes. |
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