pH-dependence of 13C chemical shifts and 13C,H coupling constants in imidazole and L-histidine. |
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Authors: | R E Wasylishen and G Tomlinson |
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Abstract: | The pH-dependence of selected 13C chemical shifts reflects the state of ionization of the imidazole ring in both imidazole and L-histidine. Titration of the amino and carboxyl groups of histidine also perturbs the shifts. The coupling constants 1J (13C(2),H) and 1J (13C(5),H) for both compounds also vary with pH, but in L-histidine these constants are relatively insensitive to the titration of groups outside the imidazole ring. |
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