Influence of putrescine on anthocyanin production in callus cultures of <Emphasis Type="Italic">Daucus carota</Emphasis> mediated through calcium ATPase |
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Authors: | Sudha?G Email author" target="_blank">Ravishankar?G?A?Email author |
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Institution: | (1) Department of Plant Cell Biotechnology, Central Food Technological Research Institute, 570 013 Mysore, India |
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Abstract: | The influence of Putrescine (Put) on the growth and elicitation of anthocyanin in callus cultures of Daucus carota var. Nantes scarlet was investigated through the use of α-DL-difluoromethylarginine (DFMA), the polyamine (PA) biosynthetic
inhibitor. It was observed that the addition of Put (0.05 mM) resulted in enhancement of growth and anthocyanin content. The
anthocyanin content was found to be enhanced by 1.68 fold on the 21st day as compared to the untreated controls. The PA inhibitor was found to result in lowering of the growth and the anthocyanin
accumulation, which could be partially restored by the addition of Put in combination with this inhibitor. The levels of Ca2+ ATPase were also found to be elevated in treatment with Put suggesting the involvement of calcium in the elicitation of anthocyanin.
The endogenous titres of PAs and the ethylene production under these treatments were also studied. The treatment with DFMA
resulted in lower levels of endogenous PAs and higher levels of ethylene. Lowering of ethylene by putrescine treatment shows
that PA treatment also inhibited ethylene formation, which would also imply that endogenous ethylene does not influence anthocyanin
production in carrot callus cultures. |
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Keywords: | anthocyanin DFMA DFMO polyamines putrescine |
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