小鼠海马锥体细胞树突棘形态的电镜三维重建

大多数神经元的复杂三维结构是很难直接观察的。激光扫描共聚焦显微镜技术结合染料标记技术可以重建神经元的三维形态，但精细结构的识别需要电子显微镜。利用透射电子显微镜技术，可以得到连续超薄组织切片的高分辨率图像，结合计算机支持的三维重建技术就可进一步获得神经细胞精细结构的三维信息。通过电镜三维重建技术对未成熟和成熟小鼠海马锥体细胞树突棘的形态进行了观察和分析，并对其关键步骤的操作技巧进行了重点说明。实验结果为进一步利用成像技术研究树突棘的结构、功能和可塑性提供了重要信息。

ELECTRON MICROSCOPIC 3D-RECONSTRUCTION OF MOUSE HIPPOCAMPAL PYRAMIDAL DENDRITIC SPINES

The complex three-dimensional (3D) shapes of most neurons are difficult to observe. With laser scanning confocal microscopy, the shapes of labeled neurons in three dimensions can be reconstructed rapidly, but identification of subtle structures requires electron microscopy. Through serial sections electron microscopy and computer-based SD-reconstruction technique subtle 3D structure of neurons can be obtained. EM 3D-reconstruction technique was introduced, and the key procedures and skills were emphasized. Using this technique, the dendritic spines of pyramidal cell in hippocampus of both young and mature mice were analyzed. The results provide important information for the study of structure, function and plasticity of dendritic spines.