小麦高分子量谷蛋白亚基效应的比较研究

采用SDS-PAGE方法，通过对5个亲本间杂交获得的F2群体每一单株的F3籽粒样本及其亲本进行小麦高分子量谷蛋白亚基（HMW-GS）组成分析，并对每一F2单株上F3籽粒群体的高分子量谷蛋白亚基组成与其籽粒蛋白质含量、SDS-沉降值的关系进行研究，分析比较黄淮麦区出现频率较高的7个亚基或亚基对的品质效应。结果表明：小麦高分子量谷蛋白亚基组成不同群体间籽粒的蛋白质含量和SDS-沉降值基本达到显著或极显著水平。优质亚基表现为：1、7＋8、14＋15和5＋10亚基。因此，黄淮麦区小麦育种应加强对这些优质亚基的引入和利用，特别是对14＋15和5＋10亚基的引入和利用。

Comparison of the Effects of High Molecular Weight Glutenin Subunits in Wheat

The HMW-GS compositions of the sampled F_3 kernels and parental kernels of each plant in the F_2 populations formed by crossing five parents were assayed by SDS-PAGE.The relationships of the HMW-GS compositions of the F_3 kernels of each F_2 plants with the protein contents and SDS-sedimentation value of these kernels were studied and seven high-frequency subunits and their quality effects in Huanghuai Area of Wheat Production were comparatively probed.The results showed that different populations of wheat with different HMW-GS compositions by and large appeared to significantly or extremely significantly differ in the protein contents and SDS-sedimentation values of their kernels.The quality subunits or subunit combinations were subunit 1,subunit 7+subunit 8,subunit 14+subunit 15 and subunit 5+subunit 10.The therefore,more attention should be paid to the introduction and exploitation of these subunit or subunit combinations in wheat breeding for Huanghuai Area of Wheat Production.