Application of lectin microarray to crude samples: differential glycan profiling of lec mutants |
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Authors: | Ebe Youji Kuno Atsushi Uchiyama Noboru Koseki-Kuno Shiori Yamada Masao Sato Takashi Narimatsu Hisashi Hirabayashi Jun |
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Institution: | Nano/Bio Science Research Lab., Yokohama Technical Center, Moritex Corporation, 1-3-3 Azamino-Minami, Aoba-ku, Yokohama, Kanagawa 225-0012. |
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Abstract: | We recently developed a novel system for lectin microarray based on the evanescent-field fluorescence-detection principle, by which even weak lectin-oligosaccharide interactions are detectable without a washing procedure. For its practical application, cell glycan analysis was performed for Chinese hamster ovary (CHO) cells and their glycan profile was compared with those of their glycosylation-defective Lec mutants. Each of the cell surface extracts gave a significantly different profile from that of the parental CHO cells in a manner reflecting denoted biosynthetic features. Hence, the developed lectin microarray system is considered to be fully applicable for differential glycan profiling of crude samples. |
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