Proton translocation in intact cells of Thiobacillus denitrificans |
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Authors: | Olli H Tuovinen D J Donald Nicholas M I Hussain Aleem |
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Institution: | (1) Department of Agricultural Biochemistry Waite Agricultural Research Institute, The University of Adelaide, 5064 Glen Osmond, South Australia, Australia;(2) Thomas Hunt Morgan School of Biological Sciences, University of Kentucky, 40506 Lexington, KY, USA;(3) Present address: Department of General Microbiology, University of Helsinki, Malminkatu 20, SF-00100 Helsinki 10, Finland |
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Abstract: | Proton translocation assessed by the quinacrine fluorescence technique was compared with oxygen uptake during thiosulphate oxidation by cells of Thiobacillus denitrificans. The addition of thiosulphate to cell suspensions resulted in an outwardly directed proton translocation as reflected by an increased quinacrine fluorescence. Compared to the O2 uptake activity, the proton translocating system was much more sensitive to proton conductors, other ionophores and inhibitors of electron transport. The results indicate that (a) the proton-translocation activity (membrane energization) is enhanced in aged cell suspensions, (b) intactness of the cytoplasmic membrane is essential for establishing a protonmotive force in cells, (c) the fluorescence increase and proton translocation are reversible processes, (d) inhibitors of electron transport may also act as proton conductors by altering the integrity of the cytoplasmic membrane.Abbreviations CCCP
carbonyl cyanide m-chlorophenyl-hydrazone
- DBP
2,4-dibromophenol
- DNP
2,4-dinitrophenol
- HOQNO
2-heptyl-4-hydroxyquinoline-N-oxide
- PCP
pentachlorophenol
- TPB
tetraphenyl boron
- TTFA
1-thenoyl-(2)]-3,3,3-trifluoracetone |
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Keywords: | Thiobacillus denitrificans Proton translocation Electron transport Oxygen uptake Fluorescence emission Throsulphate oxidation Membrane energization |
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