| 应用热冲击处理技术提高发酵甘油的产率 |
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| 引用本文： | 谢东明 刘德华 张岩 朱丙田 刘天中 苏志国. 应用热冲击处理技术提高发酵甘油的产率[J]. 生物工程学报, 2000, 16(3): 383-385 |
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| 作者姓名： | 谢东明 刘德华 张岩 朱丙田 刘天中 苏志国 |
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| 作者单位： | 中国科学院化工冶金研究所生化工程国家重点实验室北京 100080 |
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| 基金项目： | 国家“九五”攻关项目(96-C03-03-03A)，国家重点工业性实验项目。 |
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| 摘 要： | 当甘油发酵进入细胞指数生长期的中段时，对发酵体系在45℃下进行大约30 min的热冲击处理，这种处理可以显著提高发酵甘油的产率，但对细胞生长和残糖消耗的影响不大。
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| 关 键 词： | 甘油，发酵，热冲击 |
| 文章编号： | 1000-3061(2000)03-0383-04 |
| 修稿时间： | 1999-07-21 |
Enhancement of Fermentative Glycerol Yield with Heat Shock Treatment |
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| XIE Dong-Ming,LIU De-hua,ZHANG Yan,ZHU Bing-tian,LIU Tian-Zhong,SU Zhi-guo. Enhancement of Fermentative Glycerol Yield with Heat Shock Treatment[J]. Chinese journal of biotechnology, 2000, 16(3): 383-385 |
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| Authors: | XIE Dong-Ming LIU De-hua ZHANG Yan ZHU Bing-tian LIU Tian-Zhong SU Zhi-guo |
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| Affiliation: | State Key Lab of Biochemical Engineering, Chinese Academy of Sciences, Beijing. |
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| Abstract: | A heat shock treatment was studied in glycerol fermentation of osmotolerant yeast Candida krusei. The experiment results showed that the optimal temperature and duration time for heat shock is 45 degrees C and 30 min respectively, and the optimal start time of the treatment is at the mid term in exponential growth stage. With such treatment, glycerol yield was enhanced greatly, while no significant effects on both cell growth and glucose consumption were observed. |
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| Keywords: | Glycerol fermentation heat shock treatment |
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