In vitro Culture of Immature Cotyledons of Soya Bean (Glycine max L. Merr.) |
| |
Authors: | THOMPSON J F; MADISON J T; MUENSTER ANNA-MARIA E |
| |
Institution: | U.S. Plant, Soil and Nutrition Laboratory, Agricultural Research Service, U.S. Department of Agriculture Ithaca, New York 14853, U.S.A. |
| |
Abstract: | An in vitro procedure promoting the rapid growth and proteinincrease of soya bean cotyledons has been developed. The amountof protein synthesized varied greatly depending on the nitrogen(N) source provided. Glutamine was the most effective N source,while inorganic forms of N were ineffective. Growth and proteinsynthesis were both more rapid in vitro than in vivo. Underthe best conditions, soya bean cotyledons increased 8-fold bothin dry weight and in protein in 6 days. The formation of the7S and 11S storage proteins in vitro was similar to that invivo. Hence, this in vitro culture method is appropriate forstudying legume seed storage protein synthesis under controlledconditions. |
| |
Keywords: | |
本文献已被 Oxford 等数据库收录! |
|