肝片吸虫精子发生中的合体群团方式

本文从细胞形态学和超微结构等方面研究肝片吸虫Fasciola sp.精子发生的合体群团方式。结果表明:精巢直接涂片,经细胞培养、染色体观察和定向切片电镜观察,均证实肝片吸虫精子发生从B型精原细胞、初级精母细胞、次级精母细胞、精细胞直到精子排出前的最后阶段等各期生殖细胞均以由细胞间桥连接的合体群团方式存在。有倍性群团和非倍性群团两种。未见到子细胞数目超过32的群团,非倍性群团应是部分精母细胞和精细胞退化的结果。无脊椎动物肝片吸虫合体群团超微结构与前人在哺乳类中的观察相似。表明精子发生中各子细胞间是否具有由细胞间桥连接而形成的合体群团,是动物生殖细胞发生与体细胞增殖的重要区别,似是有性生殖的一种功能适应。

THE SYNCYTIAL CLUSTER FORMS IN SPERMATOGENESIS IN FASCIOLA SP.

The present study was earned out to investigate the development of syncytial cluster forms in spermatogenesis of flukes from cytomorphological, chromosome behavioral and ultrastructu-ral aspects. Light and electron microscopic examination of directional sections of testis smears showed that syncytial cluster forms existed in every stage of fluke spermatogenesis from spermatogonia type B, to spermatocytes 1 and 2, to spermatids, and to the excretion of matured sperms. There were multiple and non-multiple clusters. Clusters formed by more than 32 daughter cells have not been found. Non-multiple clusters maybe is the result of degeneration of spermarocytes and spermstids. The forms and structures of syncytial clusters in such kind of inverbebrate as flukes are similar to those of mammals. The important difference between the division of male germ cells and somatic cells is whether there are clusters formed by daughter cells connected with intercellular bridges or not probably it is a sort of function of the reproduction of male germ cells.