Use of the primed in situ labelling (PRINS) technique for a rapid detection of chromosomes 13, 16, 18, 21, X and Y |
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| Authors: | Franck Pellestor Anne Girardet Geneviève Lefort Brigitte Andréo Jean Paul Charlieu |
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| Institution: | (1) CNRS UPR, 9008, Route de Mende, BP 5051, F-34033 Montpellier Cedex, France;(2) Cytogenetics Laboratory, St. Charles Hospital, F-34295 Montpellier Cedex 5, France;(3) MRC Human Genetics Unit, Western General Hospital, Crewe Road, EH4 2XU Edinburgh, UK |
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| Abstract: | The primed in situ labelling (PRINS) technique is an alternative to in situ hybridization for chromosomal screening. We have developed a semi-automatic PRINS protocol, using a programmable thermocycler. The method has been successfully tested with specific primers for chromosomes, 13, 16, 18, 21, X and Y. Specific chromosome detection has been obtained on both metaphases and interphase nuclei. This suggests that PRINS may be a reliable technique for detecting aneuploidies and some chromosomal aberrations. |
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