仙客来(Cyclamen persicum Mill.)的种质资源RAPD分析

利用RAPD技术对20个现今栽培的名优仙客来品种的分类和亲缘关系进行研究,从100个随机引物中筛选出18个用于PCR反应,共在153个位点上扩增出条带,平均每个引物扩增位点8.5个,多态性位点144个,占总带数的94.1%。这种多肽性可以进行品种的鉴定,聚类分析将各品种材料分为4个类群。提出仙客来除目前的观赏性状分类以外,还应具有更科学的以遗传基础为基准的分类方法。也从分子水平揭示了目前栽培的仙客来品种的遗传基础的狭窄性。对RAPD技术在仙客来育种的进一步应用也作了相应的探讨。

RAPD ANALYSIS OF CYCLAMEN PERSICUM GERMPLASM

RAPD analysis was applied to Cyclamen (Cyclamen persicum Mill.)in order to assess the classification and genetic relationships among 20 cultivars of Cyclamen 18 stochastic selected from 100 ones were used to PCR reaction bands were amplified in 153 dots in all. Each primer amplified 8.5 dots ,144 poly in orphism dots equally . These dots were 94.1% of all .This polymorphism could identify cultivars .Every cultivar resource was set apart into 4 groups by the analysis of grouped together . Cyclamen should has more scientific classified method based on gene besides ornamental character at present. The result also revealed Cyclamen cultivar's stricture of genetic base in the level of molecule .The farther application of RAPD in Cyclamen had been discussed.