Epitope mapping of a single repetitive unit of the B13 Trypanosoma cruzi antigen as fusions to Escherichia coli LamB protein |
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| Authors: | Pereira Cátia M Franco da Silveira José Duranti Márcia Cunha-Neto Edécio Zingales Bianca Castilho Beatriz A |
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| Institution: | 1. Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, UNIFESP, Rua Botucatu 862, CEP 04023-062 São Paulo, SP, Brazil;2. Departamento de Bioqu??mica, Instituto de Qu??mica, Universidade de São Paulo, São Paulo, Brazil;3. Laboratório de Imunologia de Transplantes, Instituto do Coração, Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil;1. Laboratorio de Tecnología Inmunológica, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina;2. Servicio de Clínica Médica, Hospital J.B. Iturraspe, Santa Fe, Argentina;3. Cátedra de Química Analítica Clínica, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Santa Fe, Argentina;4. Servicio de Cardiología, Hospital Provincial del Centenario, Carrera de Especialización en Cardiología, Facultad de Ciencias Médicas, Universidad Nacional de Rosario, Santa Fe, Argentina;5. Departamento de Parasitología Celular y Molecular, Escuela de Medicina, Universidad de Juntendo, Tokio, Japón;6. Instituto de Inmunología, Facultad de Medicina, Universidad Nacional de Rosario, Santa Fe, Argentina;1. University of Tampere, Institute of Medical Technology, Tampere, Finland;2. Tampere University Hospital, Tampere, Finland;3. University of Helsinki, Department of Dermatology and Venereal Diseases, Helsinki, Finland;1. Institute of Neuroimmunology, Slovak Academy of Sciences, 842 45 Bratislava, Slovakia;2. SISSA-ISAS, International School for Advanced Studies, Trieste, Italy;3. Bioscience Division, Los Alamos National Laboratory, MS-M888,Los Alamos, NM 87545, USA;4. DanubeClone s.r.o. 842 45, Bratislava, Slovakia;5. Universita'' degli Studi Tor Vergata, Rome, Italy |
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| Abstract: | B13, one of the immunodominant antigens of Trypanosoma cruzi, is composed of repeats of a 12-amino-acid motif. Using synthetic peptides, the sequence FGQAAAGDK was previously shown to contain the B13 immunodominant epitope recognized by chagasic patients sera. To investigate the effects of neighboring sequences in the immunodominance, we tested serum recognition of two B13 sequences fused to LamB. GDKPSPFGQAAA-LamB and FGQAAAGDKPSP-LamB were recognized, respectively, by 15% and 80% of 80 sera reactive to B13 antigen. Recognition of FGQAAAGDKPSP-LamB was inhibited by AAAGDK-containing synthetic peptides. FGQAAAGDKPSP-LamB competed with a B13 recombinant protein containing 16.6 repeats for binding to chagasic antibodies. These results strengthen previous conclusions on the immunodominant epitope of B13 and provide a comparison of two methods for epitope mapping. |
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| Keywords: | Trypanosoma cruzi Immunodominant repetitive antigen B13 antigen B cell epitope mapping Escherichia coli LamB protein |
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