In vitro micropropagation of Acacia nilotica subsp. indica Brenan via cotyledonary nodes |
| |
Authors: | Anita Dewan Kanan Nanda Shrish C. Gupta |
| |
Affiliation: | (1) Department of Botany, University of Delhi, 110007 Delhi, India;(2) Department of Botany, Daulat Ram College, University of Delhi, India |
| |
Abstract: | Cotyledonary node explants of Acacia nilotica subspecies indica Brenan, differentiated multiple shoots on Gamborg et al.' s medium (B5, Gamborg et al. 1968) supplemented with cytokinins like N6-benzyladenine, 6-(, -Dimethylallylamino)-purine, kinetin or zeatin. Of the four, BA supported maximum multiple shoot differentiation; the highest average number of shoots (6.3) per expiant was in 1.5 mg/l. The number of shoots was further enhanced by (i) using nodal explants of in vitro regenerated shoots as microcuttings, and (ii) repeated subculture of the original expiants (stumps) on the same medium after excising the shoots. Thus, over seven hundred shoots could be obtained from a single cotyledonary node explant. Individual shoots, when transferred to 2 mg/l indole-3-acetic acid augmented medium organised healthy roots in 100% cultures. Such test tube grown plantlets have been successfully transferred to soil, where they grow well up to eight weeks.Abbreviations 2iP 6-(, -Dimethylallylamino)-purine - AC activated charcoal - B5 Gamborg et al. 's medium with 0.8% agar + 3 % sucrose - BA,N6 benzyladenine - CW coconut water - FAA formalin-aceticacid-alcohol - IAA indole-3-acetic acid - IBA indole-3-butyric acid - Kn kinetin - NAA -naphthaleneacetic acid - NOA -naphthoxyacetic acid |
| |
Keywords: | Acacia nilotica in vitro regeneration cotyledonary node leguminous tree |
本文献已被 SpringerLink 等数据库收录! |
|