Distribution of myosin and the glial fibrillary acidic protein (GFA Protein) in rat spinal cord and in the human frontal cortex as revealed by immunofluorescence microscopy |
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| Authors: | Dr. E. Braak D. Drenckhahn K. Unsicker U. Gröschel-Stewart D. Dahl |
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| Affiliation: | (1) Department of Anatomy, University of Kiel, Kiel, Germany;(2) Department of Anatomy, University of Kiel, D-2300 Kiel, Federal Republic of Germany;(3) Department of Zoology, TH Darmstadt, Darmstadt, Germany;(4) Department of Neuropathology, Harvard Medical School, Boston, Mass., USA |
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| Abstract: | Summary The glial fibrillary acidic (GFA) protein and myosin were localized in rat spinal cord and human frontal cortex using specific antibodies against GFA protein from human spinal cord and highly purified smooth myosin from chicken gizzard by means of an indirect immunofluorescence microscopical approach. A strong GFA protein and myosin immunoreactivity was found in astrocytes of the white and grey matter and in the external glial limitans membrane. The very fine branches of astrocytic processes stained with antiGFA protein, but not with anti-myosin. Similar results were obtained with the human frontal cortex, where myosin antibodies failed to reveal the very fine branches of protoplasmic astrocytes.As a whole, staining with the GFA protein antiserum was more crisp than with the myosin antibody.Thanks are due to Professor J.R. Wolff, Max-Planck Institute for Biophysical Chemistry, Göttingen, for stimulating discussions, to Ursula König, Christa Mahlmeister and Renate Steffens for skilful technical assistance, and to Heidi Waluk for the photographic workSupported by grants from Deutsche Forschungsgemeinschaft (Br 634/1, Dr 91/1, Un 34/4, Ste 105/19)Dedicated to Prof. Dr. med. H. Leonhardt on the occasion of his 60. birthday |
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| Keywords: | Glial cells Myosin GFA protein Central nervous tissue (rat, human) Immunofluorescence microscopy |
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